Monoclonal Antibody to Aurora A Kinase (Clone: 1A11)

Name: Monoclonal Antibody to Aurora A Kinase (Clone: 1A11)
Brand: Abeomics
SKU: 34-1011
Price: 462.0000 USD
Availability: InStock
Rating: 4.4 (10 reviews)

Figure-1: Immunofluorescent analysis of HeLa cell cultures stained with mouse mAb to aurora A kinase,(34-1011), in green, and costained with chicken pAb to vimentin,(34-1126), in red. The blue is DAPI staining of nuclear DNA. (34-1011) antibody labels aurora A protein expressed in spindle poles at late mitosis.

Monoclonal Antibody to Aurora A Kinase (Clone: 1A11)

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Figure-1: Immunofluorescent analysis of HeLa cell cultures stained with mouse mAb to aurora A kinase,(34-1011), in green, and costained with chicken pAb to vimentin,(34-1126), in red. The blue is DAPI staining of nuclear DNA. (34-1011) antibody labels aurora A protein expressed in spindle poles at late mitosis.

Figure-2: Western blot analysis of different cell lysates and recombinant protein solutions using mouse mAb to aurora A,(34-1011). Left: cells were treated with 100ng/mL of nocodazol (a microtubule depolymerizing agent which induces cells to halt at G2/M phase) for 6 hours: [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] Equine NBL6 cells, and [5] murine KR158 cells. The antibody apparently does not recognize canine or equine aurora A, but does bind human and murine. Right: equal amounts of purified human recombinant aurora A, B, C as indicated. The (34-1011) antibody reacts with the aurora A recombinant protein but not aurora B or C.

Product code: 34-1011

Clone name :	1A11
Clonality :	Monoclonal
Application :	WB
Reactivity :	Human

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For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	T.C. Sup.
Amount :	500 µl
Isotype :	Mouse, IgG1
Content :	Antibody is supplied as an aliquot of concentrated tissue culture supernatant.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	AURKA
Gene ID :	6790
Uniprot ID :	O14965
Alternative Name :	Aurora 2, ARK-1, Aurora/IPL1-related kinase 1, Breast tumor-amplified kinase, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase aurora-A
Immunogen Information :	Full length recombinant human proteinÂ aurora A expressed in and purified from E. coli

WB: 1:100-1:500. ICC/IF and IHC: 1:100-1:500.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Cytoplasm, Cytoplasm, Cytoplasm
Post transnational modification:	Ubiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL7) during mitosis, leading to its degradation by the proteasome. Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
Tissue Specificity:	Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
BioGrid:	112666. 374 interactions.