Monoclonal antibody to ATG5 (Clone: ABM5A86)
Figure-1: Western blot analysis of ATG5. Anti-ATG5 antibody (Clone: ABM5A86) was used at 4 µg/ml on (1) 3T3 (2) PANC-1, (3) A431, (4) K562, (5) PC3 and (6) HCT-116 lysates.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Mouse IgG1, Kappa |
| Purification : | Protein G Chromatography |
| Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
Recommended dilutions: WB: 4-6 µg/ml. However, this need to be optimized based on the research applications.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Preautophagosomal structure membrane |
| Post transnational modification: | Acetylated by EP300. |
| Tissue Specificity: | Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. |
| BioGrid: | 114859. 41 interactions. |
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