Anti-PCNA Monoclonal Antibody (Clone: PC 10)
Figure 1: Anti-PCNA antibody(monoclonal) 39-1086. Western blotting: Lane 1: MCF-7 Cell Lysate, Lane 2: HELA Cell Lysate, Lane 3: HT1080 Cell Lysate, Lane 4: COLO320 Cell Lysate.
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| Amount : | 100 μg/vial |
| Isotype : | Mouse IgG2a |
| Purification : | Ascites |
| Content : | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. Reconstitute : Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Storage condition : | At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing. |
Proliferating cell nuclear antigen(PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.
Western blot : 2μg/ml; Immunohistochemistry(Paraffin-embedded Section) : 0.4-1μg/ml; Immunohistochemistry(Frozen Section) : 0.4-1μg/ml; Immunocytochemistry : 1μg/ml;
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus |
| Post transnational modification: | Methylated on glutamate residues by ARMT1. |
| BioGrid: | 247765. 3 interactions. |
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