Anti-Nebulin Monoclonal Antibody (Clone: NB2)
Figure 1: Anti-Nebulin antibody 39-1074. Western blotting: Lanes: Anti Nebulin(39-1074) at 0.5ug/ml; WB: Mouse Skeletal Muscle Tissue Lysate at 50ug. Predicted band size: 773 kDa. Observed band size: 773 kDa.
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| Amount : | 100 μg/vial |
| Isotype : | Mouse IgG1 |
| Purification : | Ascites |
| Content : | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. Reconstitute : Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Storage condition : | At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing. |
Nebulin is a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800 kD in a manner that is tissue-, species-, and developmental stage-specific. The nebulin gene contains 183 exons in a 249-kb genomic region. Nebulin is mapped to chromosome 2. Nebulin is a giant filamentous protein specific for vertebrate skeletal muscles. Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy.
Western blot : 1-2 μg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Cytoplasm |
| Tissue Specificity: | Muscle specific. Located in the thin filament of striated muscle. |
| BioGrid: | 110783. 25 interactions. |
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