Anti-Fibronectin Monoclonal Antibody (Clone: FN-15)
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| Amount : | 100 μg/vial |
| Isotype : | Mouse IgG1 |
| Purification : | Ascites |
| Content : | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. Reconstitute : Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Storage condition : | At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing. |
| Gene : | Fn1 |
| Uniprot ID : | P04937 |
| Alternative Name : | Fibronectin; FN; Anastellin; Fn1 |
| Immunogen Information : | Fibronectin from human plasma. |
Fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Cellular fibronectin is a 550KDa glycoprotein existing as two heterodimers, different from plasma fibronectin in the presence of additional polypeptide segments and in altering morphology of transformed cells and hemaglutination.
Western blot : 1-2μg/ml; Immunohistochemistry(Paraffin-embedded Section) : 1-2μg/ml; Immunohistochemistry(Frozen Section) : 1-2μg/ml; Immunocytochemistry : 1μg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Secreted |
| Post transnational modification: | Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation. |
| Tissue Specificity: | Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. |
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