Anti-Cytokeratin Peptide 18 Monoclonal Antibody (Clone: CY-90)
Figure 1: Anti-Cytokeratin Peptide 18 monoclonal antibody(39-1024) . Western blotting: MCF-7 Cell Lysate.
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| Amount : | 100 μg/vial |
| Isotype : | Mouse IgG1 |
| Purification : | Ascites |
| Content : | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Storage condition : | At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing. |
Intermediate filaments(IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18(KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.
Western blot : 0.5-1μg/ml; Immunohistochemistry(Paraffin-embedded Section) : 1-2μg/ml; Immunohistochemistry(Frozen Section) : 0.5-2μg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus matrix, Nucleus, Cytoplasm |
| Post transnational modification: | O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. |
| Tissue Specificity: | Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue. |
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