Anti-alpha-Smooth Muscle Actin Monoclonal Antibody (Clone: 1A4)
Figure 1: Anti-alpha-Smooth Muscle Actin antibody(monoclonal) 39-1056. IHC(P): Rat Cardiac Muscle Tissue.
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| Amount : | 100 μg/vial |
| Isotype : | Mouse IgG2a |
| Purification : | Ascites |
| Content : | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. Reconstitute : Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Storage condition : | At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing. |
Ueyama et al.(1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.
Western blot : 2μg/ml; Immunohistochemistry(Paraffin-embedded Section) : 0.1-0.5μg/ml; Immunohistochemistry(Frozen Section) : 0.1-0.5μg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm |
| Post transnational modification: | Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration (By similarity). |
| BioGrid: | 249533. 1 interactions. |
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