MMLV RT Recombinant Protein
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| Amount : | 10000,U |
| Content : | 50mM Tris-HCl, 0.1M NaCl, 0.1% Triton X-100, 2mM DTT, 0.1mM EDTA and 50% glycerol. |
| Storage condition : | Stable for 5 days at 10°C, for longer period of time store at -20°C. Please prevent freeze-thaw cycles. |
Source : Recombinant E. coli strain.
MMLV (Moloney Murine Leukemia Virus) Reverse Transcriptase is a DNA polymerase that synthesizes a complementary DNA strands from single-stranded RNA, DNA, or an RNA-DNA hybrid as a template. This recombinant enzyme was purified from E.coli, which carried a modified MMLV-RT gene. Compared to AMV Reverse Transcriptase, this enzyme has a much weaker 5' - 3' ribonuclease H activity, which allows the syntesis of longer cDNAs (>7kb).
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