IGFBP4 Recombinant Protein
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| Amount : | 20 µg |
| Purification : | Greater than 95.0% as determined by SDS-PAGE. |
| Content : | IGFBP 4 was lyophilized after extensive dialysis against PBS. |
| Storage condition : | Lyophilized IGFBP4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFBP-4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| AA sequence : | DEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGELDCHQ LADSFRE + His Tag. |
| Alternative Name : | Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4, IGFBP4, IBP4, BP-4, HT29-IGFBP. |
Source : HEK293 cells.
IGFBP-4 Human Recombinant amino acids Asp22- Glu258, produced in HEK293 cells and fused with a polyhistidine tag at the C-terminus. IGFBP4 predicted Mw is 27kDa and on SDS-PAGE appears as a 32kDa band under denaturing conditions.IGFBP4 is purified by proprietary chromatographic techniques.
It is recommended to reconstitute the lyophilized IGFBP4 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. The ED50 range is 0.01-0.09µg/ml as measured by its ability to inhibit the biological activity of IGFII (20ng/ml) on MCF7 human breast adenocarcinoma cells.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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