Human TIMP-2 (Metalloproteinase Inhibitor-2) Pre-Coated ELISA Kit

This assay employs an antibody specific for Anti-human TIMP-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TIMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This immunoassay kit allows for the in vitro quantitative determination of Human TIMP-2 concentrations in serum, plasma and other biological fluids.

Detection Range: 0.156 – 10 ng/ml

Sensitivity: < 0.094 ng/ml

For Research Use Only. Not for use in diagnostic/therapeutics procedures.