Human PD-1 (Programmed Cell Death Protein1) Pre-Coated ELISA Kit

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human PD-1 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human PD-1 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PD-1 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human PD-1 can be calculated using the standard curve.

This immunoassay kit allows for the in vitro quantitative determination of Human PD-1 concentrations in serum, plasma and other biological fluids.

Detection Range: 0.156 – 10 ng/ml

Sensitivity: < 0.094 ng/ml

For Research Use Only. Not for use in diagnostic/therapeutics procedures.