Human IFN Alpha Pre-Coated ELISA Kit

This assay employs an antibody specific for Anti-human IFN-α coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN-α present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IFN-α antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN-α bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. 

This immunoassay kit allows for the in vitro quantitative determination of Human IFN-α concentrations in serum, plasma and other biological fluids.

Detection Range: 15.6 – 1000 pg/ml

Sensitivity: <9.4 pg/ml

For Research Use Only. Not for use in diagnostic/therapeutics procedures.