Human HSP gp96 (Heat Shock Protein Glycoprotein 96) Pre-Coated ELISA Kit(Discontinued)

This assay employs an antibody specific for Anti-human HSP gp96 coated on a 96-well plate. Standards and samples are pipetted into the wells and HSP gp96 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human HSP gp96 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HSP gp96 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This immunoassay kit allows for the in vitro quantitative determination of Human HSP gp96 concentrations in serum, plasma and other biological fluids. 

Detection Range: 0.313 – 20 ng/ml

Sensitivity: < 0.188 ng/ml

For Research Use Only. Not for use in diagnostic/therapeutics procedures.