HLA-DRA Recombinant Protein
Figure 1: Western Blot Analysis. Loaded 5ug of protein, using 1:5000 dilution of Anti-His HRP.
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| Amount : | 20 µg |
| Purification : | "Greaterthan 90% as determined by SDS-PAGE." |
| Content : | HLA-DRA protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Storage condition : | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| AA sequence : | MGSSHHHHHH SSGLVPRGSH MIKEEHVIIQ AEFYLNPDQS GEFMFDFDGD EIFHVDMAKK ETVWRLEEFG RFASFEAQGA LANIAVDKAN LEIMTKRSNY TPITNVPPEV TVLTNSPVEL REPNVLICFI DKFTPPVVNV TWLRNGKPVT TGVSETVFLP REDHLFRKFH YLPFLPSTED VYDCRVEHWG LDEPLLKHWE FDAPSPLPET TE |
| Alternative Name : | Major Histocompatibility Complex, Class II, DR Alpha, HLA-DRA1, MHC Class II AntigenDRA, MLRW, Histocompatibility AntigenHLA-DR Alpha, HLAClass II Histocompatibility Antigen, DR Alpha Chain, MHC Cell Surface Glycoprotein, HLA-DRA1. |
Source : Escherichia Coli. HLA-DRA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 212 amino acids (26-216 a.a) and having a molecular mass of 24.3kDa.HLA-DRA is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Major histocompatibility complex, class II, DR alpha (HLA-DRA) belongs to the MHC class II family. HLA-DRA binds peptides derived from antigens which access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for identification by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mainly by degradation of proteins which access the endocytic route, where they are processed by lysosomal proteases and other hydrolases.
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