GATC Recombinant Protein
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| Amount : | 10 µg |
| Purification : | Greater than 85.0% as determined by SDS-PAGE. |
| Content : | GATC protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 30% glycerol and 1mM DTT. |
| Storage condition : | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| AA sequence : | MGSSHHHHHH SSGLVPRGSH MGSMWSRLVW LGLRAPLGGR QGFTSKADPQ GSGRITAAVI EHLERLALVD FGSREAVARL EKAIAFADRL RAVDTDGVEP MESVLEDRCL YLRSDNVVEG NCADELLQNS HRVVEEYFVA PPGNISLPKL DEQEPFPHS |
| Alternative Name : | Glutamyl-TRNA(Gln) Amidotransferase Subunit C, Glu-AdT Subunit C, 15E1.2, Glutamyl-TRNA(Gln) Amidotransferase Subunit C Homolog (Bacterial), Glutamyl-TRNA(Gln) Amidotransferase Subunit C Mitochondrial, Glutamyl-TRNA(Gln) Amidotransferase Subunit C Ho |
Source : Escherichia Coli.
GATC Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 159 amino acids (1-136 a.a) and having a molecular mass of 17.5kDa (Molecular size on SDS-PAGE will appear higher).GATC is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Glutamyl-TRNA
Amidotransferase, Subunit C also known as GATC allows the formation of properly
charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in
the mitochondria. The reaction occurs in the attendance of glutamine and ATP through
an activated gamma-phospho-Glu-tRNA(Gln). In addition, GATC is subunit of the
heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112)
and C (GATC) subunits.
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