EPO a His Recombinant Protein
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Amount : | 10 µg |
Purification : | Greater than 80% as determined by SDS-PAGE. |
Content : | EPO a protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. |
Storage condition : | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
AA sequence : | MGSSHHHHHH SSGLVPRGSH MGSAPPRLIC DSRVLERYLL EAKEAENITT GCAEHCSLNE NITVPDTKVNFYAWKRMEVG QQAVEVWQGL ALLSEAVLRG QALLVNSSQP WEPLQLHVDK AVSGLRSLTT LLRALRAQKEAISPPDAASA APLRTITADT FRKLFRVYSN FLRGKLKLYT GEACRTGDR. |
Alternative Name : | Erythropoietin, EP, INN=Epoetin, EPO a, Erythropoietin-alpha, EPO. |
Source : Escherichia Coli.
EPO a Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 189 amino acids (28-193a.a.) and having a molecular mass of 20.9kDa.EPO a is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
EPO a is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. EPO a is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. EPO a also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
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