DDT Recombinant Protein
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Amount : | 25 µg |
Purification : | Greater than 95.0% as determined by SDS-PAGE. |
Content : | DDT Human solution containing 20mM Tris HCL pH-8, & 10% glycerol. |
Storage condition : | DDT Human although stable at 4°C for 1 week, should be stored desiccated below -18°C. Please prevent freeze thaw cycles. |
AA sequence : | MGSSHHHHHH SSGLVPRGSH MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF FPLESWQIGK IGTVMTFL. |
Alternative Name : | EC 4.1.1.84, DDCT, D-dopachtome decarboxylase, D-Dopachrome Tautomerase. |
Source : Escherichia Coli.
DDT Recombinant E.coli produced in E.Coli is a single, non-glycosylated polypeptide chain containing 138 amino acids (1-118 a.a.) and having a molecular mass of 14.8 kDa. The DDT is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
DDT is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihydroxyindole (DHI). DDT is part of the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. DDT shares a homologous amino acid sequence (33% identical) with MIF and has similar tautomerase activity. DDT functions a proinflammatory cytokine.
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