COVID-19 nucleoprotein ELISA Kit

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody (monoclonal) was precoated onto 96-well plates. And the biotin conjugated antibody(monoclonal) was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer.HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

This immunoassay kit detects quantitative detection of nucleoprotein in serum, plasma, tissue homogenates, cell culture

supernatant, cell culture lysate and other biological fluids(throat swab, nose swab).