Coronavirus (COVID-19) Nucleocapsid Antibody
Figure-1: Wells of a 96-microtiter plate were coated with 4ug/ml of SARS-Cov-2/nCov/COVID-19 Nucleocapsid recombinant protein (21-1003). The binding was detected by addition of different dilution of 11-2003 polyclonal antibody. The reactivity was detected by a HRP-conjugated goat-anti-rabbit polyclonal antibody.
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG |
| Purification : | Protein A Chromatography |
| Content : | 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage condition : | Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles. |
| Immunogen Information : | A partial length recombinant coronavirus Nucleocapsid protein (amino acids 250-410) was used as the immunogen for this antibody. |
The structural nucleocapsid (N) protein of nCoV/SARS-CoV-2/COVID-19 is a predicted 46 kDa phosphoprotein having 419 amino acid residues. A short Serine rich stretch and a recognized nuclear localization signal are the unique features of the nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19, which seems to have a little homology with the proteins of other members of this large corona virus family. These features also suggest the involvement of nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 in different stages of viral lifecycle. The protein has multifaceted activities including packing of the nCoV/SARS-CoV-2/COVID-19 viral genome into a helical ribonucleocapsid (RNP) and playing an important role in viral self-assembly causing nCoV/SARS-CoV-2/COVID-19. The nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 also forms dimer in the cell by self-association with the help of interactive C terminal domain.
Recommended dilutions: WB: 0.5-1 µg/ml. However, this need to be optimized based on the research applications.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Shradha Mawatwal, Amruta Mohapatra, Sayani Das, Aisurya Ray, Ratnadeep Mukherjee, Gulam Syed, Shantibushan Senapati, Balachandran Ravindran
Prior exposure to malaria decreases SARS-CoV-2 mediated mortality in K18-hACE2
mice without influencing viral load in lungs
Section for Immunology, Division of Infection Control, Norwegian Institute of Public
Health, Oslo, Norway
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