Biotinylated Recombinant Rabbit Monoclonal Antibody to Mouse IgG2a (Clone: RM219)(Discontinued)
Figure 1: ELISA of mouse immunoglobulins shows Clone: RM219 reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA,IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/ml, 50 ng/ml, or 10 ng/ml of Clone: RM219 was usedas the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondaryantibody.
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| Format : | Purified |
| Amount : | 50 µg |
| Isotype : | Rabbit IgG |
| Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
| Content : | 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
Clone RM219 reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. ELISA: 0.005 µg/ml – 0.2 µg/ml; Immunocytochemistry (ICC): 0.5 µg/ml-2 µg/ml; Immunohistochemistry (IHC): 0.5 µg/ml-2 µg/ml; Western Blot (WB): 0.5 µg/ml-2 µg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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