Biotinylated Recombinant Rabbit Monoclonal Antibody to GAPDH (Clone: RM114)(Discontinued)
Figure 1: Immunocytochemical staining of HeLa cells, using anti-GAPDH Clone: RM114 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).
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| Format : | Purified |
| Amount : | 50 µl |
| Isotype : | Rabbit IgG |
| Purification : | Protein A affinity purified from an animal origin-free culture supernatant |
| Content : | 1 mg/ml in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Storage condition : | Store at -20°C. Avoid repeated freeze and thaw cycles. |
This antibody reacts to GAPDH. Western Blot (WB): 1:1000 dilution; Immunoprecipitation (IP): 1:200 dilution; Chromatin IP (ChIP): 1:200 dilution; Immunocytochemistry (ICC): 1:200 dilution; Immunohistochemistry (IHC): 1:200 dilution.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Nucleus, Cytoplasm, Membrane, Cytoplasm |
| Post transnational modification: | Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation. |
| BioGrid: | 108868. 233 interactions. |
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