Anti-TNF alpha Biotin
ELISA analysis of TNF alpha (0 - 4.1 ng/ml) using capture antibody MAb1 (5 μg/ml) and biotinylated detection antibody MAb11 (0.15 - 10 μg/ml).
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| Amount : | 0.1 mg |
| Isotype : | Mouse IgG1 kappa |
| Purification : | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
| Content : | 1 mg/ml Formulation : Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Storage condition : | Store at 2-8°C. Do not freeze. |
Specificity : The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha). TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
ELISA: Biotinylated MAb11 can be used as a detection antibody in combination with capture antibody MAb1.
Immunohistochemistry (frozen sections): paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Flow cytometry: Intracellular staining.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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