Anti-Perforin APC Mab (Clone:dG9)

Product code: 30-2878

Clone name : dG9
Clonality : Monoclonal
Application : FACS
Reactivity : Human, Bovine
Conjugate : APC

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100 tests
$468.00 

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Amount : 100 tests
Isotype : Mouse IgG2b kappa
Purification : Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Content : Formulation : Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage condition : Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Gene : PRF1
Gene ID : 5551
Uniprot ID : P14222
Alternative Name : PRF1, P1, PFP, HPLH2
Immunogen Information : purified granules from human YT lymphoma cell line

Specificity: The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.

Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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