Anti-p53 Purified
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| Format : | Purified |
| Amount : | 0.1 mg |
| Purification : | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| Content : | Concentration: 1 mg/ml Storage Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Storage condition : | Store at 2-8°C. Do not freeze. |
The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.
Specificity :The antibody BP53-12 recognizes a defined epitope (aa 16-25) on human p53, a 50 kDa intracellular tumour suppressor found in increased amounts in a wide variety of transformed cells; it is frequently mutated or inactivated in many types of cancer.
Western blotting: Recommended dilution: 1-2 µg/ml, overnight at 4°C; positive control: RAMOS human lymphoma cell line, non-reducing conditions. SDS-PAGE (12% separating gel).
Immunohistochemistry: Recommended dilution: 5-10 µg/ml.
Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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