Anti-NTAL / LAB Monoclonal Antibody (Clone:NAP-03)

NTAL (Non-T cell Activation Linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.

Immunoprecipitation Western Blotting Recommended dilution: 1-2 µg/ml, incubation 60 min
Positive control: RAMOS human lymphoma cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Application note: Non-Reducing conditions. 12% separating SDS-PAGE gel.
Immunohistochemistry Recommended dilution: 5 µg/ml
Positive tissue: tonsil, germinal center

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cell membrane
Post transnational modification:	May be polyubiquitinated.
Tissue Specificity:	Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level).
BioGrid:	113301. 4 interactions.

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