Anti-Neisseria meningitidis antigen Orf1 / FrpD Polyclonal Antibody
Figure 1: Western Blotting analysis (reducing conditions) of recombinant protein Orf1 in cell lysate of Orf1-transfected E. coli using polyclonal anti-Orf1 antibody.
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Rabbit IgG |
| Purification : | Purified from rabbit serum by affinity chromatography |
| Storage condition : | Store at 2-8°C. Do not freeze. |
| Immunogen Information : | Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit. |
Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.
Western Blotting Recommended dilution: 0.2 - 1 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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