Anti-Lyn Monoclonal Antibody (Clone:LYN-01)
Figure 1: Western Blotting analysis (non-reducing conditions) of Lyn using anti-Lyn (LYN-01). Lane 1: negative control. Lane 2: RBL rat basophilic leukemia cell line . Lane 3: JURKAT human peripheral blood T cell leukemia cell line . Lane 4: A-431 human epidermoid carcinoma cell line . Lane 5: U-937 human histiocytic lymphoma cell line
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Mouse IgG1 |
| Purification : | Purified by protein-A affinity chromatography |
| Storage condition : | Store at 2-8°C. Do not freeze. |
Lyn is a Src-family protein tyrosine kinase that is predominantly expressed in hematopoietic cells. It is associated with a number of cell surface receptors including the B cell antigen receptor (BCR) and Fc receptors. Upon their triggering, Lyn phosphorylates subunits of these receptors in a cholesterol-dependent manner, utilizing the plasma membrane lipid raft system. The phosphorylated intracellular domains of the receptors are accessible for cytoplasmic Syk tyrosine kinase, which is activated by Lyn-mediated phosphorylation and which transduces the signal to downstream adaptors. Lyn is abnormally distributed in acute myeloid leukemia cells and seems to be a novel pharmacologic target of this disease.
Western Blotting Recommended dilution:
1-2 µg/ml, 60 min
Positive control:
RBL rat basophilic leukemia cell line
JURKAT human peripheral blood T cell leukemia cell line
A-431 human epidermoid carcinoma cell line
U-937 human histiocytic lymphoma cell line
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cell membrane, Nucleus, Cytoplasm, Cytoplasm, Golgi apparatus, Membrane |
| Post transnational modification: | Autophosphorylated. Phosphorylated on tyrosine residues in response to KIT signaling. Phosphorylation at Tyr-397 is required for optimal activity. Phosphorylation at Tyr-508 inhibits kinase activity. Phosphorylated at Tyr-508 by CSK. Dephosphorylated by PTPRC/CD45. Becomes rapidly phosphorylated upon activation of the B-cell receptor and the immunoglobulin receptor FCGR1A. |
| Tissue Specificity: | Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors. |
| BioGrid: | 110245. 199 interactions. |
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