Anti-LIME Monoclonal Antibody (Clone:LIME-10)
Figure 1: Western Blotting analysis (reducing conditions) of human LIME using anti-human LIME (LIME-10). Lane 1: J77 cell line transfected with LIME. Lane 2: non-transfected J77 cell line (cells are essentialy devoid of endogenous LIME expression)
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Mouse IgG2a |
| Purification : | Purified by protein-A affinity chromatography |
| Storage condition : | Store at 2-8°C. Do not freeze. |
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
| Subcellular location: | Cell membrane |
| Post transnational modification: | Phosphorylated on tyrosines upon TCR activation and/or CD4 coreceptor stimulation, or upon BCR stimulation; which leads to the recruitment of SH2-containing proteins. |
| Tissue Specificity: | Expressed in peripheral blood lymphocytes, lymphoid tissues, and liver. Present in T-cells and plasma cells, and in various hematopoietic cell lines (at protein level). |
| BioGrid: | 120264. 4 interactions. |
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