Anti-Involucrin (Squamous Cell Terminal Differentiation Marker) Recombinant Rabbit Monoclonal Antibody (Clone:IVRN/2113R)
Figure 1: Formalin-fixed, paraffin-embedded human Tonsil stained with Involucrin Rabbit Recombinant Monoclonal Antibody (IVRN/2113R).
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Format : | Purified |
Amount : | 100 µg |
Isotype : | Rabbit IgG |
Purification : | Protein A/G |
Content : | 200µg/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
Does not react with mouse.
Flow Cytometry (0.5-1µg/million cells); ,Immunofluorescence (1-2µg/ml); ,Immunohistology (Formalin-fixed) (0.1-0.2µg/ml for 30 min at RT),(Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37°C),(Note than enzyme digestion is better than citrate buffer based epitope unmasking),Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Cytoplasm |
Post transnational modification: | Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. |
Tissue Specificity: | Keratinocytes of epidermis and other stratified squamous epithelia. |
BioGrid: | 109917. 40 interactions. |
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