Anti-Human CD315 MAb (Clone: 1F11) Purified
Fig 1: Separation of A431 cells stained using anti-human CD315 (1F11) purified antibody (concentration in sample 1.67 μg/ml, GAM APC) from A431 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Mouse IgG1 |
| Purification : | Purified by protein-A affinity chromatography. |
| Content : | 1 mg/ml Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Storage condition : | Store at 2-8°C. Do not freeze. |
Specificity : The mouse monoclonal antibody 1F11 recognizes an extracellular epitope of CD315, a type I transmembrane glycoprotein expressed on keratinocytes, activated monocytes, and a subset of B cells.
CD315, also known as prostaglandin F2 receptor negative regulator, is an approximately 135 kDa transmembrane glycoprotein, which associates with actin cytoskeleton, and with CD9 and CD81, but not with other tetraspanins. It seems to be involved in regulation of cell polarity and motility. CD315 is expressed mainly by keratinocytes, activated monocytes, and a subset of B cells, but it can be also used for distinguishing between strongly positive colon cancer and fibrosarcoma cells, and their negative normal cell counterparts.
Flow cytometry: Recommended dilution: 1-4 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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