Anti-Human CD1d PE MAb (Clone: 51.1)
Fig1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD1d (51.1) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
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| Amount : | 100 tests (T100) |
| Isotype : | Mouse IgG2b kappa |
| Purification : | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Content : | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide. |
| Storage condition : | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Specificity: The mouse monoclonal antibody 51.1 recognizes an extracellular epitope of CD1d, a 38 kDa transmembrane glycoprotein expressed mainly on cortical thymocytes, marginal zone B cells and other antigen presenting cells, but also i e.g. hepatitis C virus-infected livers.
CD1d belongs to CD1 family of transmembrane glycoproteins, associated with beta2 microglobulin, similarly to MHC I molecules. Unlike other CD1 family members, however, CD1d can be also expressed in a non-glycosylated form, which is not associated with beta2 microglobulin. Hence it is not certain how much CD1d plays a role in the presentation of microbial lipid antigens during infection. On the other hand, it is expressed on various antigen presenting cell types. Besides it, CD1d+ thymocytes are involved in the positive selection of sublineage of NKT cells.
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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