Anti-Hu CD77 FITC
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| Amount : | 100 Tests |
| Purification : | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Content : | Storage Buffer: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| Storage condition : | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Alternative Name : | globotriaosylceramide Gb3, Gal-alpha1-4Gal-beta1-4Glc-beta1-Cer, P blood group |
| Immunogen Information : | Daudi cell line (Burkitt´s lymphoma) |
CD77 (globotriaosylceramide Gb3), also known as the Pk blood group antigen, BLA (Burkitt´s lymphoma associated antigen), or CTH (ceramide trihexoside) is a neutral glycosphingolipid composed of three carbohydrate molecules linked to a lipid moiety in the cell membrane (Gal-alpha1-4Gal-beta1-4Glc-beta1-Cer). It is expressed on germinal center B cells, Burkitt´s lymphoma cells, it can be induced on extrafolicular B cells and it is also found on endothelia and epithelia. CD77 may be involved in elimination of germinal center B cells that fail to produce high affinity antibodies, and serves also as receptor for shiga toxin and verotoxin.
Specificity :The rat monoclonal antibody 38.13 recognizes CD77 (globotriaosylceramide Gb3), a neutral glycosphingolipid expressed mainly on the surface of B cell populations, such as germinal center B cells and Burkitt´s lymphoma cells.
Specificity :The rat monoclonal antibody 38.13 recognizes CD77 (globotriaosylceramide Gb3), a neutral glycosphingolipid expressed mainly on the surface of B cell populations, such as germinal center B cells and Burkitt´s lymphoma cells.
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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