Anti-Hu CD326 Purified
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| Format : | Purified |
| Amount : | 0.1 mg |
| Purification : | Purified by protein-A affinity chromatography. |
| Content : | Concentration: 1 mg/ml Storage Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Storage condition : | Store at 2-8°C. Do not freeze. |
CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.
Specificity :The mouse monoclonal antibody VU-1D9 recognizes an extracellular epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.
Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml; positive control: colon epithelium, antigen retrieval: heat (sodium citrate) + trypsin. <br>Immunocytochemistry: Recommended dilution: 4 μg/ml. <br>Western blotting: Recommended dilution: 1-4 μg/ml, non-reducing conditions. <br> Flow cytometry: Recommended dilution: 1-3 μg/ml. <br>Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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