Anti-Hu CD173 PE Mab(MEM-195)
Figure 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD173 (MEM-195) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
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Amount : | 100 Tests |
Isotype : | Mouse IgM |
Purification : | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Content : | Formulation: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
Storage condition : | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Alternative Name : | Lewis Y, blood group H2 antigen, LeY |
Immunogen Information : | Human thrombocytes |
Specificity: The antibody MEM-195 reacts with CD173 (H2), an extracellular saccharide antigen expressed mainly during early hematopoiesis; it is also expressed on endothelial cells.
CD173 (blood group antigen H2) is a fucosylated saccharide (Fuc-alpha-1-2-Gal-beta-1-4-GlcNAc-beta) generated by beta-D-galactoside 2-alpha-L-fucosyltransferase (FUT1). CD173 belongs to markers of early hematopoiesis; it is expressed mainly on CD34-positive hematopoietic progenitor cells. CD173 is a precursor structure of CD174 (Lewis Y) and is also structurally related to CD15 (Lewis X). On endothelial cells CD173 and CD174 are concentrated on pseudopodial extensions responsible for initial contacts between endothelial cells.
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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