Azide free Anti-HLA-G Monoclonal Antibody (Clone:MEM-G/9)
Figure 1: Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) and protein G. HLA-G was detected by anti-human HLA-G (4H84; ) and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).
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| Amount : | 0.1 mg |
| Isotype : | Mouse IgG1 |
| Purification : | Purified by protein-A affinity chromatography |
| Storage condition : | Store at 2-8°C. Do not freeze. |
Flow cytometry: Recommended dilution: 0.3-4 μg/ml; positive control: JEG-3 human choriocarcinoma cell line. <br>Immunocytochemistry: Recommended dilution: 2-5 μg/ml. <br>ELISA: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody 10 μg/ml, detection antibody (biotin or peroxidase conjugate) 1 μg/ml. <br>Immunohistochemistry: Recommended dilution: 1-4 μg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Secreted |
| Tissue Specificity: | Expressed in trophoblasts. Expressed in fetal eye and thymus (PubMed:2336406). Also expressed in adult eye (PubMed:1570318). Isoform 4: Expressed in fetal first trimester trophoblasts (PubMed:7589701). Isoform 7: Expressed in first trimester, second trimester and term placenta, fetal liver, amniotic membrane, skin, cord blood and peripheral blood mononuclear cells (PubMed:11137219). |
| BioGrid: | 109380. 18 interactions. |
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