Anti-HLA-DR Monoclonal Antibody (Clone:MEM-12)
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Mouse IgG1 |
| Purification : | Purified by protein-A affinity chromatography |
| Storage condition : | Store at 2-8°C. Do not freeze. |
HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.
Flow Cytometry Recommended dilution:2 µg/ml
Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line
Immunoprecipitation Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line
Western Blotting Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Do not heat/boil.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cell membrane, Endoplasmic reticulum membrane, Golgi apparatus, Endosome, Lysosome |
| Post transnational modification: | N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
| BioGrid: | 107410. 13 interactions. |
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