Anti-Helios Monoclonal Antibody (Clone:22F6)
Figure 1: Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Helios (22F6) purified antibody (concentration in sample 8 μg/ml, GAM APC).
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Hamster IgG |
| Purification : | Purified by protein-A affinity chromatography |
| Storage condition : | Store at 2-8°C. Do not freeze. |
Helios, also known as IKZF2 (Ikaros family zinc finger protein 2) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Ikaros. Helios forms homo- and heterodimers with these proteins and is thought to function predominantly in early hematopoietic development. Expression of Helios, Aiolos and Ikaros is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Helios is expressed at early stages of thymocyte development. In mature T cells, Helios has been strongly associated with Treg cells.
Flow Cytometry Staining method:
- Perform staining of cell surface markers (CD25, CD4 etc.) for 20 min. at room temperature in the dark; 100 µl of peripheral blood.
- Add 3 ml of PBS with 1% BSA, centrifugate at 300g and discard the supernatant. Further steps perform on ice and with ice-cold reagents.
- Resuspend the cells in 5 ml of cold fixation solution (Miltenyi Biotec) and incubate for 30 min. on ice.
- Centrifugate for 5 min. at 1000 g, 4
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus |
| BioGrid: | 116485. 20 interactions. |
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