Anti-GFP VHH antibody, biotinylated
Fig 1: Indirect ELISA showing anti-GFP VHH antibody, biotinylated(JOT0001-2) binding to purified GFP. Plates were coated with 100ng/well purified protein and binding of JOT0001-2 assessed in serial dilution from 1ng/ml primary antibody in triplicate
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| Amount : | 50 µg |
| Isotype : | Camelid VHH |
| Purification : | Affinity chromatography purified via Ni-charged resin |
| Content : | Format: Liquid Product Buffer Solution: 25 mM TAPS pH8.5, 500 mM NaCl, 5 mM EDTA, 0.1 % Proclin 300 Solution Product Concentartion : 1 mg/mL by Nanodrop |
| Storage condition : | 4°C; Do not freeze. |
| Gene : | GFP |
| Uniprot ID : | P42212 |
| Alternative Name : | Green fluorescent protein |
Specificity: Green fluorescent protein (GFP)
Description : Alpaca derived anti-GFP VHH single domain antibody, biotinylated (molecular weight: 15.82 kDa), expressed in E. coli under conditions free from animal derived components.
Conjugate and tags : A 6*His tag at its C-terminal, followed by an Avi tag. The single lysine residue in the Avitag is enzymatically labeled with one biotin.
Analyte Description : Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr (previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488 nm.
This is a product from Jotbody, Hong Kong. This antibody is made available worldwide by ABEOMICS Inc.
ELISA 1:9000-1:200000
WB 1:500-1:5000
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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