Anti-GFAP VHH antibody
Figure 1: Lysate of U87-MG cell line was subjected to SDS- PAGE followed by western blot with anti-GFAP VHH antibody (JOT0052-1) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
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| Amount : | 50 µg |
| Isotype : | Camelid VHH |
| Purification : | Affinity chromatography purified via Ni-charged resin. Purity : > 95% as determined by SDS-PAGE |
| Content : | 1 mg/mL by Nanodrop Buffer : 25 mM TAPS pH8.5, 500 mM NaCl, 5 mM EDTA, 0.1 % Proclin 300 |
| Storage condition : | 4°C; Do not freeze. |
Alpaca derived anti-GFAP VHH single domain antibody (molecular weight:13.6 kDa) with a 6*His tag at its C-terminal, expressed in E. coli under conditions free from animal derived components.
Glial fibrillary acidic protein (GFAP) is a class III intermediate filament protein that is largely expressed in astrocytes in addition to non-myelinating Schwann cells and glial cells. Human GFAP protein is 432 amino acids in length with a theoretical molecular weight of ~50 kDa. GFAP has at least 10 known isoforms, with the most prevalent and common in the brain being GFAP-alpha which is made of a head domain, a rod domain with four coils (1A, 1B, 2A, 2B) joined by linker regions, and a tail domain. GFAP is a marker of mature astrocytes, but is also expressed throughout development in both fetal and adult neural stem cells. While the exact function of GFAP is still elusive, it has been shown to play a role in cellular processes such as migration, mitosis, structural integrity, and signalling.
Specificity: Glial Fibrillary Acidic Protein (GFAP)
This is a product from Jotbody, Hong Kong. This antibody is made available worldwide by ABEOMICS Inc.
Positive controls : Positive WB detected in: U87-MG cells
Positive IHC detected in: mouse brain tissue <> Recommended dilutions : WB 1:1000-1:5000
IHC: 1:200-1:600
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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