Anti-GAPDHS Monoclonal Antibody (Clone:Hs-8)
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Mouse IgM |
| Purification : | Purified by precipitation and chromatography |
| Storage condition : | Store at 2-8°C. Do not freeze. |
| Gene : | GAPDHS |
| Gene ID : | 26330 |
| Uniprot ID : | O14556 |
| Alternative Name : | GAPDHS, GAPD2, GAPDH2, GAPDS, HSD-35, HSD35 |
| Immunogen Information : | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Western Blotting Immunocytochemistry Recommended dilution:
Purified Antibody: 10 µg/ml
Staining technique: Membrane permeabilization (acetone) is essential.
Flow Cytometry
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm |
| Tissue Specificity: | Testis specific. |
| BioGrid: | 117681. 56 interactions. |
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