Anti-GAPDHS FITC (Clone : Hs-8)
Figure 1 : Flow cytometry detection of GAPDHS in acrosomes of human sperms with anti-GAPDHS (Hs-8) FITC.
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| Amount : | 50 tests |
| Isotype : | Mouse IgM |
| Purification : | The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| Content : | Formulation : Stabilizing Tris buffered saline (TBS) solution with 15 mM sodium azide |
| Storage condition : | Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. |
| Gene : | GAPDHS |
| Gene ID : | 26330 |
| Alternative Name : | SGAPDH, HSD-35,glyceraldehyde-3-phosphate dehydrogenase, spermato |
| Immunogen Information : | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Specificity : The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.
Specificity : The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 50 tests.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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