Anti-EGFR (Phospho-Tyr992) Monoclonal Antibody (Clone:EM-12)

Figure 1: Western blotting analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).

Anti-EGFR (Phospho-Tyr992) Monoclonal Antibody (Clone:EM-12)

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Figure 1: Western blotting analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).

Figure 2: Immunoprecipitation of EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.

Figure 3: Immunoprecipitation of EGFR from EGF-treated A431 cells by phosphospecific monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.

Product code: 30-1399

Clone name :	EM-12
Clonality :	Monoclonal
Application :	FACS
Reactivity :	Human

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Download TDS MSDS

Format :	Purified
Amount :	0.1 mg
Isotype :	Mouse IgG1
Purification :	Purified by protein-A affinity chromatography
Storage condition :	Store at 2-8°C. Do not freeze.


Gene :	EGFR
Gene ID :	1956
Uniprot ID :	P00533
Alternative Name :	EGFR, ERBB, ERBB1, HER1
Immunogen Information :	Synthetic phospho-peptide covering sequence around tyrosine 992 of human EGFR.

The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.

Immunoprecipitation Western Blotting Recommended dilution: 1 µg/ml
Positive control: EGF-stimulated A431
Negative control: Non-stimulated A431

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Secreted
Post transnational modification:	Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
Tissue Specificity:	Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
BioGrid:	108276. 1217 interactions.