Anti-EGFR (Phospho-Tyr1173) Monoclonal Antibody (Clone:EM-13)
Figure 1: Western blotting analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reducing conditions).
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Mouse IgG1 |
| Purification : | Purified by protein-A affinity chromatography |
| Storage condition : | Store at 2-8°C. Do not freeze. |
The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.
Immunoprecipitation Western Blotting Recommended dilution: 1 µg/ml
Positive control: EGF-stimulated A431
Negative control: Non-stimulated A431
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Secreted |
| Post transnational modification: | Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197. |
| Tissue Specificity: | Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. |
| BioGrid: | 108276. 1217 interactions. |
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