Anti-Cytokeratin 5/8 Monoclonal Antibody (Clone:C-50)
Figure 1:Western Blotting analysis of whole cell lysate of MCF-7 human breast adenocarcinoma cell line. Lane 1: MW standard. Lane 2: immunostaining with anti-cytokeratin 5/8 (clone C-50). Lane 3: immunostaining with Isotype mouse IgG1 control (PPV-06; )
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Mouse IgG1 |
| Purification : | Purified by protein-A affinity chromatography |
| Storage condition : | Store at 2-8°C. Do not freeze. |
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Immunohistochemistry Recommended dilution:10 µg/ml
Positive control:Human prostate
Immunoprecipitation Western Blotting
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Nucleus |
| Post transnational modification: | O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. |
| Tissue Specificity: | Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. |
| BioGrid: | 110073. 113 interactions. |
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