Anti-Cytokeratin 18 Monoclonal Antibody (Clone:DC-10)-Biotin Conjugated

Figure-1: Flow cytometry analysis (intracellular staining) of HeLa cells with anti-human cytokeratin 18 (DC-10) biotin antibody (red, concentration in sample 6 Î¼g/ml) streptavidin-APC, with blank sample (black).

Anti-Cytokeratin 18 Monoclonal Antibody (Clone:DC-10)-Biotin Conjugated

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Figure-1: Flow cytometry analysis (intracellular staining) of HeLa cells with anti-human cytokeratin 18 (DC-10) biotin antibody (red, concentration in sample 6 Î¼g/ml) streptavidin-APC, with blank sample (black).

Figure-2: Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody DC-10 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of biotinylated mouse anti-cytokeratin 18 monoclonal antibody followed by IRDye800-conjugated streptavidin. Cytokeratin 18 was detected at approximately 46 kDa.

Product code: 30-1827

Clone name :	DC-10
Clonality :	Monoclonal
Application :	IP
Reactivity :	Human
Conjugate :	Biotin

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Download TDS MSDS

Amount :	0.1 mg
Isotype :	Mouse IgG1
Storage condition :	Store at 2-8°C. Do not freeze.


Gene :	KRT18
Gene ID :	3875
Uniprot ID :	P05783
Alternative Name :	KRT18, CYK18, PIG46
Immunogen Information :	Human breast carcinoma cell line PMC-42.

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Cytokeratin 18 belongs to type I family (acidic cytokeratins).

Flow cytometry: Recommended dilution: 2-6 μg/ml. Intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/ml.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Nucleus
Post transnational modification:	O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
Tissue Specificity:	Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
BioGrid:	110073. 113 interactions.