Anti-Cytokeratin 18 Monoclonal Antibody (Clone:DA-7)

Figure 1:Western Blotting analysis (reducing conditions) of whole cell lysate using anti-human Cytokeratin 18 (DA-7). Lane 1: HeLa human cervix carcinoma cell line. Lane 2: A-431 human epidermoid carcinoma cell line. Lane 3: MCF-7 human breast adenocarcinoma cell line

Anti-Cytokeratin 18 Monoclonal Antibody (Clone:DA-7)

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Figure 1:Western Blotting analysis (reducing conditions) of whole cell lysate using anti-human Cytokeratin 18 (DA-7). Lane 1: HeLa human cervix carcinoma cell line. Lane 2: A-431 human epidermoid carcinoma cell line. Lane 3: MCF-7 human breast adenocarcinoma cell line

Figure 2: Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 18 (clone DA-7).

Product code: 30-1045

Clone name :	DA-7
Clonality :	Monoclonal
Application :	IP
Reactivity :	Human

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Download TDS MSDS

Format :	Purified
Amount :	0.1 mg
Isotype :	Mouse IgG1
Purification :	Purified by protein-A affinity chromatography
Storage condition :	Store at 2-8°C. Do not freeze.


Gene :	KRT18
Gene ID :	3875
Uniprot ID :	P05783
Alternative Name :	KRT18, CYK18, PIG46
Immunogen Information :	Human breast carcinoma cell line PMC-42.

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Cytokeratins 18 belongs to type I family (acidic cytokeratins).

Flow cytometry: Recommended dilution: 1-4 μg/ml. Extracellular and intracellular staining.

Western blotting: Recommended dilution: 1-2 μg/ml.Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: colon, skin. Immunocytochemistry: Positive control: MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line. ELISA: Positive control: MCF-7 human breast adenocarcinoma cell line; the antibody DA-7 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm, Nucleus
Post transnational modification:	O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
Tissue Specificity:	Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
BioGrid:	110073. 113 interactions.