Anti-Cyclin E (G1/S-Phase Cyclin) Monoclonal Antibody(Clone: CCNE1/2460)
Fig. 1: Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cyclin E Mouse Monoclonal Antibody (CCNE1/2460).
Roll over image to zoom in
Shipping Info:
For estimated delivery dates, please contact us at [email protected]
| Amount : | 100 µg |
| Isotype : | Mouse IgG2b, kappa |
| Content : | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress throµgh S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|
There are currently no product reviews
|
.png "ACE2/HEK293 Stable Cell Line")
