Anti-Cyclin D1 Monoclonal Antibody (Clone:CD1.1)
Figure 1: Separation of MCF-7 cells stained using anti-Cyclin D1 (CD1.1) purified antibody (GAM APC, red-filled) from MCF-7 cells stained using mouse IgG1 isotype control (MOPC-21) purified antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Mouse IgG1 |
| Purification : | Purified by protein-A affinity chromatography |
| Storage condition : | Store at 2-8°C. Do not freeze. |
Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.
Flow Cytometry Application note: Membrane permeabilization is required.
Immunoprecipitation Recommended dilution: 1 µg/ml
Western Blotting Recommended dilution: 1 µg/ml
Immunohistochemistry Pretreatment: Heat treatment, sodium citrate buffer pH 6.0.
Immunohistochemistry (frozen sections) Recommended dilution: 2 µg/ml
Positive tissue: colon
Immunocytochemistry Recommended dilution: 1 µg/ml
ELISA
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus, Cytoplasm, Membrane |
| Post transnational modification: | Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex. |
| Tissue Specificity: | Ubiquitous. Up-regulated in livers of iron-overloaded patients with hereditary hemochromatosis. |
| BioGrid: | 107067. 101 interactions. |
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