Anti-Cyclin D1 Biotin MAb (Clone: DCS-6)
Figure 1: Separation of MCF-7 cells stained using anti-Cyclin D1 (DCS-6) Biotin antibody (concentration in sample 5,0 μg/ml, Streptavidin APC, red-filled) from MCF-7 cells unstained by primary antibody (Streptavidin APC, black-dashed) in flow cytometry analysis (intracellular staining).
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| Amount : | 0.1 mg |
| Isotype : | Mouse IgG2a |
| Purification : | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
| Content : | 1 mg/ml
Storage Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Storage condition : | Store at 2-8°C. Do not freeze. |
Specificity: The mouse monoclonal antibody DCS-6 recognizes cyclin D1, an ubiquitously expressed 33 kDa protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.
Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.
Flow cytometry: Recommended dilution: 2-10 μg/ml. Intracellular staining.
Immunohistochemistry: Recommended dilution: 5-10 μg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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