Anti-CD62P / P-selectin Monoclonal Antibody (Clone:HI62P)

Figure 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD62P (HI62P) purified antibody (concentration in sample 0.56 μg/ml).

Anti-CD62P / P-selectin Monoclonal Antibody (Clone:HI62P)

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Figure 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD62P (HI62P) purified antibody (concentration in sample 0.56 μg/ml).

Figure 2: Separation of human lymphocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62P (HI62P) purified antibody (concentration in sample 0.56 μg/ml).

Figure 3: Anti-Hu CD62P Purified (clone HI62P) works in WB application under reducing conditions.

Western blotting analysis was performed on RIPA buffer extracts of thrombocytes, leukocytes, and Jurkat cells mixed with hot reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody HI62P (2 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed.

CD62P was detected at ~ 125 kDa in thrombocytes and leukocytes.

Product code: 30-1311

Clone name :	HI62P
Clonality :	Monoclonal
Application :	FACS
Reactivity :	Human

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For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	0.1 mg
Isotype :	Mouse IgG1
Purification :	Purified by protein-A affinity chromatography
Storage condition :	Store at 2-8°C. Do not freeze.


Gene :	SELP
Gene ID :	6403
Uniprot ID :	P16109
Alternative Name :	SELP, GMRP, GRMP
Immunogen Information :	Human platelets

CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.

Subcellular location:	Cell membrane
Tissue Specificity:	Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
BioGrid:	112303. 9 interactions.